It should be clear by now that beating the diffraction limit is extremely difficult. Nevertheless, a lot of research in optics has been and still is directed towards realising this goal. Many attempts...It should be clear by now that beating the diffraction limit is extremely difficult. Nevertheless, a lot of research in optics has been and still is directed towards realising this goal. Many attempts have been made, some successful, others not so, but, whether successful or not, most were based on very ingenious ideas. To close this chapter on diffraction theory, we will give examples of attempts to achieve what is called super-resolution.
The study of membranes poses a unique challenge in science due to their complex and fluid nature. Until recently, the majority of experiments was conducted in either fixed samples or on population lev...The study of membranes poses a unique challenge in science due to their complex and fluid nature. Until recently, the majority of experiments was conducted in either fixed samples or on population levels that did not give insight into individual events. However, recent advances in fluorescence microscopy techniques have allowed scientists to visualize microscopic perturbations of individual vesicles and have allowed the study of membrane dynamics at higher spatiotemporal resolution than before.
