This article will focus on discussing mass spectrometry, time of flight (ToF, mass detector) and time of flight coupled with secondary ion mass spectrometry (ToF-SIMS) as a tool to study membrane lipi...This article will focus on discussing mass spectrometry, time of flight (ToF, mass detector) and time of flight coupled with secondary ion mass spectrometry (ToF-SIMS) as a tool to study membrane lipids. In recent years, mass spectrometry has been a widely used tool to analyze complex biological systems.